Here is Larial at a glance:
Larial Analytical is a protein expression, purification and analysis
company. Our core expertise is in the separation and analytical
sciences areas. The types of separation science technologies we
routinely utilize are centrifugation, electrophoresis (1 and 2d,
isoelectric focusing), affinity chromatography, HPLC and FPLC. The
analytical technologies we routinely utilize are mass spectrometry, SPR
biosensing, ELISA, analytical ultracentrifugation, Western
immunoblotting, UV/Vis spectroscopy. We are also capable of doing
NMR (nuclear magnetic resonance), SFC/SFE (supercritical fluid
chomatography and extraction) and CE (capillary electrophoresis) work. The
specific services we offer are biomarker discovery and screening, assay
design and SOP development, synthetic peptide antibody production and
protein structure/function studies.
Quick Links to Larial Services and Technology
Project Management: Scientific algorithm generation, grant writing and consultation, investor relations, SR&ED planning and application, staffing, scientific instrumentation sourcing, assay design and validation, patent filing, technical application note writing, QA/QC, due diligence, marketing/PR, competitive intelligence
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Proteomics (proteome stabilization and analysis); Biomarker Analysis (discovery, screening, validation); BioInformatics (molecular modelling, hydropathy analysis, sequence alignment, epitope identification)
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Protein Expression (E. coli, Sf9, yeast, mammalian); Peptide synthesis (immunogen, bioactive, ligand); Antibody Development (synthetic peptide, monoclonal; Protein/peptide purification (FPLC, HPLC, isoelectric focusing, salt fractionation/ion exchange chromatography, SPE, phase partitioning, affinity chromatography, preparative gel electrophoresis)
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Bioanalysis: protein conformation and interaction studies (mass spectrometry, NMR, circular dichroism, SPR biosensing, co-immunoprecipitation, gel filtration, analytical ultracentrifugation); protein purity and yield (protein assay, 1 and 2-d gel electrophoresis, RP-HPLC, Western immunoblot, ELISA),
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Here is a more detailed overview of Larial:
Scanning EM of a malarial parasite preparation
used for metabolic and proteome analysis
Larial began providing proteomics-based services in the fall of 2001 when only a few sequenced genomes including human and yeast were available in public databases. The sequenced genomes of various organisms and the commercial introduction of high resolution mass spectrometers allowed for the global analysis of proteins (the proteome) through peptide mass fingerprinting and ion fragmentation sequencing. The use of genomic information for the analysis and identification of protein expression patterns connected dynamic whole-cell and whole-organism physiology (phenotype) with the static information content of an organism's DNA (genotype). The sequenced genomes permitted the development of search engines that enabled protein identifications and post-translational modifications to be deduced with reasonable confidence. This lead to studies of the expression patterns of physiologically-related (and often co-regulated) groups of proteins, and the identification of potential regulatory mechanisms for numerous cellular biochemical processes. Comparison of amino acid sequences of homologous proteins in diverse species also lead to the rapid growth in knowledge pertaining to protein folding (Structural Proteomics) and enzymatic function (Functional Proteomics). Much of this knowledge has evolved directly from an understanding of the approximately 6000 proteins that comprise the yeast proteome.
Larial developed its core competence around 4 key phases of proteome analysis - bioinformatics-based tools for modeling protein structures and identifying functional domains, separation sciences for fractionating complex proteomes, mass spectrometry for identifying and characterizing protein expression patterns and high fidelity molecular probe development for accurate biomarker quantitation. While many academic organizations have assembled significant infrastructure to undertake large proteome analysis studies, Larial has maintained a focus on industry-relevant applications of proteomics-based technologies. For many private sector companies and some publically-funded laboratories there remains a need to outsource certain aspects of proteomics R&D work because of its complex nature and high infrastructure costs.